• B Amir Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
  • Y Javed Department of Dental Surgery, CMH, Lahore, Pakistan
  • S Hanif Emergency Department, Bahria Town International Hospital Lahore-Pakistan
  • M Tahir Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
  • MA Rana Head of Department Internal and Critical Care Medicine, Bahira Town International Hospital, Lahore, Pakistan
  • MM Hafeez Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan



Drug resistance, Mycobacterium tuberculosis, Drug susceptibility testing, infection, liquid drug susceptibility assay


The development of low-cost, fast, and reliable methods for detecting Mycobacterium tuberculosis (MTB) infection and drug susceptibility is critical for tuberculosis control. The new microscopic examination of liquid drug susceptibility assay (MODS) examines early MTB colonies in a liquid medium, which permits for more convenient diagnosis and testing of drug susceptibility. The sensitivity of MODS (91%) was superior as compared to the sensitivity of different culture methods (92%). The MGIT and MODS were used to monitor tuberculosis-positive sputum samples for isoniazid and rifampin susceptibility. Concordance between MODS and MGIT was found in 89 percent of cases. MODS are the fastest method used for diagnostic and susceptibility testing (median, 10.0 or 9 days). MODS is a fast, low-cost, responsive, and particular method for detecting and testing MTB susceptibility; it is specifically well-suited utilize in growing countries with high infection rates and a growing number of multidrug-resistant cases.


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How to Cite

Amir, B., Javed, Y., Hanif, S., Tahir, M., Rana, M., & Hafeez, M. (2021). CULTURING, IDENTIFICATION AND DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SPECIMEN . Pakistan Journal of Intensive Care Medicine, 2021(1), 5.



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