CULTURING, IDENTIFICATION AND DRUG RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SPECIMEN

B Amir; Y Javed; S Hanif; M Tahir; MA Rana; MM Hafeez

doi:10.54112/pjicm.v2021i1.5

Authors

B Amir Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
Y Javed Department of Dental Surgery, CMH, Lahore, Pakistan
S Hanif Emergency Department, Bahria Town International Hospital Lahore-Pakistan
M Tahir Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan
MA Rana Head of Department Internal and Critical Care Medicine, Bahira Town International Hospital, Lahore, Pakistan
MM Hafeez Institute of Molecular Biology & Biotechnology, University of Lahore, Lahore, Pakistan

DOI:

https://doi.org/10.54112/pjicm.v2021i1.5

Keywords:

Drug resistance, Mycobacterium tuberculosis, Drug susceptibility testing, infection, liquid drug susceptibility assay

Abstract

The development of low-cost, fast, and reliable methods for detecting Mycobacterium tuberculosis (MTB) infection and drug susceptibility is critical for tuberculosis control. The new microscopic examination of liquid drug susceptibility assay (MODS) examines early MTB colonies in a liquid medium, which permits for more convenient diagnosis and testing of drug susceptibility. The sensitivity of MODS (91%) was superior as compared to the sensitivity of different culture methods (92%). The MGIT and MODS were used to monitor tuberculosis-positive sputum samples for isoniazid and rifampin susceptibility. Concordance between MODS and MGIT was found in 89 percent of cases. MODS are the fastest method used for diagnostic and susceptibility testing (median, 10.0 or 9 days). MODS is a fast, low-cost, responsive, and particular method for detecting and testing MTB susceptibility; it is specifically well-suited utilize in growing countries with high infection rates and a growing number of multidrug-resistant cases.

References

Pio A., Luelmo, F., Kumaresan, J. and Spinaci, S. (1997). National tuberculosis programme review: experience over the period 1990-95. Bulletin of the World Health Organization, 75(6), 569.

Ali, W., Naseem, A., Hani, M., Shahzad, M. and Ali, F.M. (2010). Status of health professional awareness about resistant tuberculosis. Pak J Chest Med, 16: 4-8.

Bardarov, Jr., S., Dou, H., Eisenach, K., Banaiee, N., Ya, S. U., Chan, J., and Riska, P. F. (2003). Detection and drug-susceptibility testing of M. tuberculosis from sputum samples using luciferase reporter phage: comparison with the Mycobacteria Growth Indicator Tube (MGIT) system. Diagnostic microbiology and infectious disease, 45(1), 53-61.

Carriere, C., Riska P. F., Zimhony O., Kriakov J., Bardarov S., Burns J., Chan J., & Jacobs W. R., Jr. (1997). Conditionally replicating luciferase reporter phages: improved sensitivity for rapid detection and assessment of drug susceptibility of Mycobacterium tuberculosis. J Clin Microbiol 35, 3232–3239.

Cattamanchi, A., Davis, J.L., Worodria, W., den Boon, S., Yoo, S., et al. (2009) Sensitivity and specificity of fluorescence microscopy for diagnosing pulmonary tuberculosis in a high HIV prevalence setting. Int J Tuberc Lung Dis. 13: 1130– 1136.

Caviedes L, Lee T S, Gilman R H, et al. Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. J Clin Microbiol 2000; 38: 1203–1208.

Central, TB Division (2009) Revised National TB Control Programme Training Manual for Mycobacterium tuberculosis Culture & Drug susceptibility testing. New Delhi: Directorate General of Health Services, Ministry of Health and Family Welfare.

Davis, J.L., Huang, L., Worodria, W., Masur, H., Cattamanchi, A., et al. (2011) Nucleic acid amplification tests for diagnosis of smear-negative TB in a high HIV-prevalence setting: A prospective cohort study. PLoS One 6: e16321.

Ejigu, G. S., Woldeamanuel, Y., Shah, N. S., Gebyehu, M., Selassie, A., & Lemma, E. (2008). Microscopic-observation drug susceptibility assay provides rapid and reliable identification of MDR-TB. The International Journal of Tuberculosis and Lung Disease, 12(3), 332-337.

Elbir, H., Abdel-Muhsin, A. M., & Babiker, A. (2008). A one-step DNA PCR-based method for the detection of Mycobacterium tuberculosis complex grown on Lowenstein-Jensen media. The American journal of tropical medicine and hygiene, 78(2), 316-317.

Gandhi, N.R., Moll, A., Sturm, A.W., et al. (2006). Extensively drug-resistant tuberculosis as a cause of death in patients co-infected with tuberculosis and HIV in a rural area of South Africa. Lancet, 368: 1575–1580.

Gleissberg, V.G., Maximova, Z.D., Golubchikova, V.T., Wares, D.F. and Banatvala, N. (1999). Developing nursing practice as part of the collaborative TB control programme, Tomsk, Siberia. The International Journal of Tuberculosis and Lung Disease, 3(10), 878-885.

Jacobs, W.R., Jr., Barletta, R.G., Udani, R., Chan, J., Kalkut, G., Sosne, G., Kieser, T., Sarkis, G. J., Hatfull, G. F., and Bloom B. R. (1993). Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages. Science 260, 819–822.

Kidenya B-R, Kabangila R, Peck R-N, Mshana S-E, Webster L-E, Koenig S-P and Fitzgerald D-W (2013). Early and efficient detection of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. PloS one, 8(2), e57527.

Kiraz N, Et L, Akgun Y, Kasifoglu N, Kiremitci A (2007) Rapid detection of Mycobacterium tuberculosis from sputum specimens using the FASTPlaque TB test. Int J Tuberc Lung Dis 11: 904–908.

Kunkel, A., Zur Wiesch, P. A., Nathavitharana, R. R., Marx, F. M., Jenkins, H. E., & Cohen, T. (2016). Smear positivity in paediatric and adult tuberculosis: systematic review and meta-analysis. BMC infectious diseases, 16(1), 1-9.

Levinson W, (2010) Review of medical microbiology and immunology. The McGraw-Hill Companies.

Moore D A J, Evans C A W, Gilman R H, et al. Microscopicobservation drug susceptibility assay for the diagnosis of TB. N Engl J Med 2006; 355: 1539–1550.

Moore, D. A. J., Evans, C. A. W., Gilman, R. H., Caviedes, L., Coronel, J., Vivar, A., … Friedland, J. S. (2006). Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB. New England Journal of Medicine, 355(15), 1539–1550.

Owusu, E., & Newman, M. J. (2020). Microscopic Observation Drug Susceptibility (MODS) Assay: A Convenient Method for Determining Antibiogram of Clinical Isolates of Mycobacterium tuberculosis in Ghana. Medical Sciences, 8(1), 5.

Park, W.G., Bishai, W.R., Chaisson, R.E., Dorman, S.E. (2002). Performance of the microscopic observation drug susceptibility assay in drug susceptibility testing for Mycobacterium tuberculosis. J. Clin. Microbiol. 12, 4750–4752.

Riska P. F., & Jacobs W. R., Jr. (1998). The use of luciferase-reporter phage for antibiotic- susceptibility testing of mycobacteria. Methods Mol Biol 101, 431–455.

Steingart K-R, Sohn H, Schiller I, Kloda L-A, Boehme C-C, Pai M and Dendukuri N, (2013). Xpert® MTB/RIF assay for pulmonary tuberculosis and rifampicin resistance in adults. Cochrane database of systematic reviews, (1).

World Health Organisation. Guidelines for Surveillence of Drug Resistance in Tuberculosis. WHO/TB/2003.320-WHO/CDS/CSR/RMD/2003.3. 2003.

World Health Organization, Global Tuberculosis Program: Global Tuberculosis Control. WHO Reported (1999) 181. Geneva: WHO, 1999.